Addgene Collections

Fluorescent protein guide

Biosensors
Optogenetics
Subcellular localization
Förster resonance energy transfer (FRET)
Far-red and near-infrared
Empty backbones

Campbell lab collection

Michael Davidson collection

HHMI, Janelia and GENIE collection

Calcium ion indicators

A long Stokes shift red fluorescent Ca(2+) indicator protein for two-photon and ratiometric imaging

Wu et al. Nat. Commun., 2014. PubMed / Journal

Plasmids from this paper:

Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum

Wu et al. Biochem. J., 2014. PubMed / Journal

Plasmids from this paper:

Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response

Zhao et al. Integr. Biol. (Camb.), 2014. PubMed / Journal

Plasmids from this paper:

Improved Orange and Red Ca2+ Indicators and Photophysical Considerations for Optogenetic Applications

Wu et al. ACS Chem. Neurosci. 2013. PubMed / Journal

Plasmids from this paper:

Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry

Carlson and Campbell Prot. Eng. Des. Sel. 2013. PubMed / Journal

Plasmids from this paper:

Highlightable Ca2+ Indicators for Live Cell Imaging

Hoi et al., J. Am. Chem. Soc. 2012. PubMed / Journal

Plasmids from this paper:

An Expanded Palette of Genetically Encoded Ca2+ Indicators

Zhao et al. Science, 2011. PubMed / Journal

Plasmids from this paper:

Voltage indicators

A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices

Abdelfattah et al, J. Neurosci. 2016. Pubmed /  Journal

Plasmids from this paper:

All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins

Hochbaum, Zhao et al, Nat. Methods, 2014. Pubmed /  Journal

Plasmids from this paper:

Fluorescent proteins

An engineered monomeric Zoanthus sp. yellow fluorescent protein

Hoi et al. Chem. Biol. 2013. PubMed / Journal

Plasmids from this paper:

Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet

Carlson et al. Protein Sci. 2010. PubMed / Journal

Plasmids from this paper:

Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

Ai et al. BMC Biol., 2008. PubMed / Journal

Plasmids from this paper:

Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins

Ai et al. Biochemistry 2007. PubMed / Journal

Plasmids from this paper:

Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging

Ai et al. Biochem. J., 2006. PubMed / Journal

Plasmids from this paper:

Dimerization-dependent FPs

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. 

Ding et al. Nat. Methods 2015. PubMed / Journal

Plasmids from this paper:

 

Dimerization-dependent green and yellow fluorescent proteins

Alford et al. ACS Synth. Biol. 2012. PubMed / Journal

Plasmids from this paper:

A fluorogenic red fluorescent protein heterodimer

Alford et al. Chem. Biol. 2012. PubMed / Journal

Plasmids from this paper:

pH indicators

pHuji, a pH sensitive red fluorescent protein for imaging of exo- and endocytosis

Shen et al, J. Cell Biol., 2014. Pubmed /  Journal

Plasmids from this paper:

Red fluorescent protein pH biosensor to detect concentrative nucleoside transport

Johnson, Ai et al. J. Biol. Chem. 2009. PubMed / Journal

Plasmids from this paper:

Photoconvertible fluorescent proteins

mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities

McEvoy, Hoi, et al. PLoS ONE, 2012. PubMed / Journal

Plasmids from this paper:

Also see mMaple2 and mMaple3 from the Zhuang lab.

A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization

Hoi et al.  J. Mol. Biol., 2010. PubMed / Journal

Plasmids from this paper:

FRET-based biosensors

Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1

Belal et al. Mol. Biosyst. 2014. PubMed / Journal

Plasmid from this paper:

Forster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells

Ding et al. Anal. Chem. 2011. PubMed / Journal

Plasmids from this paper:

 

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

Ai et al. Nat. Methods. 2008. PubMed / Journal

Plasmids from this paper:

MENU