Our New Plasmids

Calcium ion indicators

Improved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications.

Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li WH, Campbell RE

ACS Chem Neurosci. 2013. PubMed / Journal

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An Expanded Palette of Genetically Encoded Ca2+ Indicators.

Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE

Science. 2011 Sep 8. PubMed / Journal

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Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response.

Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ

Integr Biol (Camb). 2014 May 19. PubMed / Journal

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Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry.

Carlson HJ, Campbell RE

Protein Eng Des Sel. 2013. PubMed / Journal

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Highlightable Ca(2+) Indicators for Live Cell Imaging.

Hoi H, Matsuda T, Nagai T, Campbell RE

J Am Chem Soc. 2012 Dec 26. PubMed / Journal

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Fluorescent proteins

An engineered monomeric Zoanthus sp. yellow fluorescent protein.

Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE

Chem Biol. 2013. PubMed / Journal

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Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins.

Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE

Biochemistry. 2007 Apr 20. ():. PubMed / Journal

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Red fluorescent protein pH biosensor to detect concentrative nucleoside transport.

Johnson DE, Ai HW, Wong P, Young JD, Campbell RE, Casey J

J Biol Chem. 2009 Jun 3. ():. PubMed / Journal

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Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet.

Carlson HJ, Cotton DW, Campbell RE

Protein Sci. 2010 Jun 2. ():. PubMed / Journal

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FRET-based biosensors

Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1.

Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE

Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e. PubMed / Journal

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Forster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells.

Ding Y, Ai HW, Hoi H, Campbell RE

Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. PubMed / Journal

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Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

Ai HW, Hazelwood KL, Davidson MW, Campbell RE

Nat Methods. 2008 May . 5(5):401-3. PubMed / Journal

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Dimerization-dependent fluorescent proteins

A fluorogenic red fluorescent protein heterodimer.

Alford SC, Abdelfattah AS, Ding Y, Campbell RE

Chem Biol. 2012 Mar 23;19(3):353-60. PubMed / Journal

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Dimerization-dependent green and yellow fluorescent proteins.

Alford SC, Ding Y, Simmen T, Campbell RE

ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. PubMed / Journal

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