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Calcium ion indicators

[/et_pb_text][et_pb_divider admin_label=”Divider” color=”#1e73be” show_divider=”on” height=”1″ /][et_pb_text admin_label=”Improved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications. 2013″ background_layout=”light” text_orientation=”left”]

Improved Orange and Red Ca Indicators and Photophysical Considerations for Optogenetic Applications.

Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li WH, Campbell RE

ACS Chem Neurosci. 2013. PubMed / Journal

Plasmids from this paper:

• CMV-CAR-GECO1
  
• CMV-R-GECO1.2
• CMV-mito-R-GECO1
• CMV-mito-CAR-GECO1
• CMV-O-GECO1

[/et_pb_text][et_pb_text admin_label=”An Expanded Palette of Genetically Encoded Ca2+ Indicators. 2011″ background_layout=”light” text_orientation=”left”]

An Expanded Palette of Genetically Encoded Ca2+ Indicators.

Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE

Science. 2011 Sep 8. PubMed / Journal

Plasmids from this paper:

• CMV-GEM-GECO1
  
• CMV-GEX-GECO1
• CMV-R-GECO1
• CMV-G-GECO1.1
• CMV-G-GECO1.2
• CMV-G-GECO1.0
• CMV-B-GECO1
• CMV-mito-GEM-GECO1
• CMV-NLS-R-GECO
• pTorPE-GEM-GECO1
• pTorPE-GEX-GECO1
• pTorPE-R-GECO1
• pTorPE-G-GECO1
• pTorPE-G-GECO1.1
• pTorPE-G-GECO1.2
• pTorPE-B-GECO1

[/et_pb_text][et_pb_text admin_label=”Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response. 2014″ background_layout=”light” text_orientation=”left”]

Microfluidic cell sorter-aided directed evolution of a protein-based calcium ion indicator with an inverted fluorescent response.

Zhao Y, Abdelfattah AS, Zhao Y, Ruangkittisakul A, Ballanyi K, Campbell RE, Harrison DJ

Integr Biol (Camb). 2014 May 19. PubMed / Journal

Plasmids from this paper:

• pTorPE-Y-GECO1
  
• CMV-Y-GECO1
• AAV-Y-GECO1
• AAV-hSyn-Lyn-Y-GECO1

[/et_pb_text][et_pb_text admin_label=”Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry. 2013″ background_layout=”light” text_orientation=”left”]

Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry.

Carlson HJ, Campbell RE

Protein Eng Des Sel. 2013. PubMed / Journal

Plasmids from this paper:

• CH-GECO2.1
• cp196V1.2

[/et_pb_text][et_pb_text admin_label=”Highlightable Ca(2+) Indicators for Live Cell Imaging. 2012″ background_layout=”light” text_orientation=”left”]

Highlightable Ca(2+) Indicators for Live Cell Imaging.

Hoi H, Matsuda T, Nagai T, Campbell RE

J Am Chem Soc. 2012 Dec 26. PubMed / Journal

Plasmids from this paper:

• pCMV-GR-GECO1.1
  
• pCMV-GR-GECO1.2
• pTorPE-GR-GECO1.1
• pTorPE-GR-GECO1.2

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Fluorescent proteins

[/et_pb_text][et_pb_divider admin_label=”Divider” color=”#1e73be” show_divider=”on” height=”1″ /][et_pb_text admin_label=”An engineered monomeric Zoanthus sp. yellow fluorescent protein. 2013″ background_layout=”light” text_orientation=”left”]

An engineered monomeric Zoanthus sp. yellow fluorescent protein.

Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE

Chem Biol. 2013. PubMed / Journal

Plasmids from this paper:

• pcDNA3.1 mPapaya1.0
• mPapaya-C1
• mPapaya-Lifeact-7
• mPapaya1-Lifeact-7
• mPapaya-N1
• mPapaya1-C1
• mPapaya1-N1
• mPapaya-pBAD

[/et_pb_text][et_pb_text admin_label=”Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins. 2007″ background_layout=”light” text_orientation=”left”]

Exploration of New Chromophore Structures Leads to the Identification of Improved Blue Fluorescent Proteins.

Ai HW, Shaner NC, Cheng Z, Tsien RY, Campbell RE

Biochemistry. 2007 Apr 20. ():. PubMed / Journal

Plasmids from this paper:

• pBad-EBFP2
• pBad-mKalama1
• pEBFP2-Nuc
• pmKalama1-Nuc

[/et_pb_text][et_pb_text admin_label=”Red fluorescent protein pH biosensor to detect concentrative nucleoside transport. 2009″ background_layout=”light” text_orientation=”left”]

Red fluorescent protein pH biosensor to detect concentrative nucleoside transport.

Johnson DE, Ai HW, Wong P, Young JD, Campbell RE, Casey J

J Biol Chem. 2009 Jun 3. ():. PubMed / Journal

Plasmids from this paper:

• pBAD-mNectarine
  

[/et_pb_text][et_pb_text admin_label=”Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet. 2010″ background_layout=”light” text_orientation=”left”]

Circularly permuted monomeric red fluorescent proteins with new termini in the beta-sheet.

Carlson HJ, Cotton DW, Campbell RE

Protein Sci. 2010 Jun 2. ():. PubMed / Journal

Plasmids from this paper:

• cp193g7
  
• cp204
• cp196
• cp201
• cp193g7N

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FRET-based biosensors

[/et_pb_text][et_pb_divider admin_label=”Divider” color=”#1e73be” show_divider=”on” height=”1″ /][et_pb_text admin_label=”Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1. 2014″ background_layout=”light” text_orientation=”left”]

Optimization of a genetically encoded biosensor for cyclin B1-cyclin dependent kinase 1.

Belal AS, Sell BR, Hoi H, Davidson MW, Campbell RE

Mol Biosyst. 2014 Feb;10(2):191-5. doi: 10.1039/c3mb70402e. PubMed / Journal

Plasmid from this paper:

• cyclin B1-CDK1 FRET biosensor
  

[/et_pb_text][et_pb_text admin_label=”Forster resonance energy transfer-based biosensors 2011″ background_layout=”light” text_orientation=”left”]

Forster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells.

Ding Y, Ai HW, Hoi H, Campbell RE

Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28. PubMed / Journal

Plasmids from this paper:

• CaYin1
  
• CaYin1 NES
• pcpmAmetrine
• pmAmetrine1.2

[/et_pb_text][et_pb_text admin_label=”Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. 2008″ background_layout=”light” text_orientation=”left”]

 

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.

Ai HW, Hazelwood KL, Davidson MW, Campbell RE

Nat Methods. 2008 May . 5(5):401-3. PubMed / Journal

Plasmids from this paper:

• pBad-mAmetrine
  
• pBad-mAmetrine1.1
• pmAmetrine-DEVD-tdTomato
• mAmetrine-C1

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Dimerization-dependent fluorescent proteins

[/et_pb_text][et_pb_divider admin_label=”Divider” color=”#1e73be” show_divider=”on” height=”1″ /][et_pb_text admin_label=”A fluorogenic red fluorescent protein heterodimer. 2012″ background_layout=”light” text_orientation=”left”]

A fluorogenic red fluorescent protein heterodimer.

Alford SC, Abdelfattah AS, Ding Y, Campbell RE

Chem Biol. 2012 Mar 23;19(3):353-60. PubMed / Journal

Plasmids from this paper:

• pDDRFP-A1
  
• pDDRFP-B1
• pDDRFP-A1B1
• pDDRFPA1-CaM
• pM13-DDRFPB1
• pDDRFP-A1B1-DEVD

[/et_pb_text][et_pb_text admin_label=”Dimerization-dependent green and yellow fluorescent proteins. 2012″ background_layout=”light” text_orientation=”left”]

Dimerization-dependent green and yellow fluorescent proteins.

Alford SC, Ding Y, Simmen T, Campbell RE

ACS Synth Biol. 2012 Dec 21;1(12):569-75. doi: 10.1021/sb300050j. Epub 2012 Aug 14. PubMed / Journal

Plasmids from this paper:

• pDDGFP-A
  
• pDDGFP-B
• pDDYFP-A
• pDDYFP-B
• pCalN-ddGFP-A
• pTOM20-ddGFP-B

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